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IRSN, Institut de radioprotection et de sûreté nucléaire

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Effects of uranium on Zebrafish reproductive function: links with biomarkers of exposure and of effects.


Congress title :SETAC Europe - 18th Annual Meeting 
Congress town :Varsovie
Congress date :25/05/2008

Document type > *Congrès/colloque

Keywords > Danio rerio, aerosol removal, radionuclides, reproductive function

Research Unit > IRSN/DEI/SECRE/LRE

Authors > AUBERGAT Lionel, BOURRACHOT Stéphanie, GILBIN Rodolphe

Publication Date > 29/05/2008

Summary

This work is part of an ongoing PhD study on the effects of radionuclides in Zebrafish, Danio rerio, a widely used freshwater fish for Ecotoxicological standard tests. Our aim is two fold: (i) to identify sensitive life stages and associated endpoints to each life history trait (from ecological parameters to biomarkers) and (ii) to evaluate their relevance for the specie life cycle. A part of this work is devoted to the study of the effects of uranium (U) on the reproductive function of adults. After 20 days of exposure to U concentrations (0, 20, 250 µg/L), the effects on ecologically relevant life history traits (laying, eggs fertilization and survival) were studied in parallel to biomarkers of exposure (ie. U bioaccumulation in the gonads and eggs/larvae) and of effects. As the radionuclides are known to induce DNA damages, we measured genotoxic effects in reproductive organs (comet assay). The effect of U on the structure and maturation of reproductive cells was also assessed through histological analysis. These results will be discussed in order to assess the usefulness of biomarkers at the tissue and/or cell level to predict effects on more relevant ecological endpoints.

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