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Human erythroid cells produced ex vivo at large scale differentiate into red blood cells in vivo.

Neildez-Nguyen TM. (1), Wajcman H. (2), Marden MC. (3), Bensidhoum M. (4), Moncollin V (1), Giarratana MC. (1), Kobari L.(1), Thierry D.(4), Douay L. (1,5) Nature Biotechnology 20(5):467-72, 2002 (1) Institut National de la Sante et de la Recherche Medicale (INSERM) U417, Hôpital Saint-Antoine, Paris, France (2) INSERM U468, Hôpital Henri Mondor, Creteil, France (3) INSERM U473, Hôpital de Bicêtre, Kremlin-Bicêtre, France (4) IRSN/DPHD/SARAM/LRH, Fontenay-aux-Roses, France (5) Service d'Hématologie Biologique, Hôpital Armand Trousseau, Paris, France

Document type > *Article de revue

Keywords > radiohematology, ex vivo expansion

Research Unit > LRTI_(Therapeutic irradiation research laboratory)

Authors > THIERRY Dominique

Publication Date > 01/03/2002

Summary

New sources of red blood cells (RBCs) would improve the transfusion capacity of blood centers. Our objective was to generate cells for transfusion by inducing a massive proliferation of hematopoietic stem and progenitor cells, followed by terminal erythroid differentiation. We describe here a procedure for amplifying hematopoietic stem cells (HSCs) from human cord blood (CB) by the sequential application of specific combinations of growth factors in a serum-free culture medium. The procedure allowed the ex vivo expansion of CD34(+) progenitor and stem cells into a pure erythroid precursor population. When injected into nonobese diabetic, severe combined immunodeficient (NOD/SCID) mice, the erythroid cells were capable of proliferation and terminal differentiation into mature enucleated RBCs. The approach may eventually be useful in clinical transfusion applications.
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