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IRSN, Institut de radioprotection et de sûreté nucléaire

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Enhancing Nuclear Safety


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A new image analysis system for biological dosimetry by fluorescent in situ hybridization. Step 1: metaphase finder and automatic metaphase acquisition validation

Roy L, Durand V, Delbos M, Sorokine-Durm I, Soussaline F, Voisin P. J Radiat Res (Tokyo) 2001 Jun;42(2):165-77

Document type > *Article de revue

Keywords > biological dosimetry, FISH

Research Unit > IRSN/DRPH/SRBE/LDB

Authors > ROY Laurence, VOISIN Philippe

Publication Date > 01/06/2001

Summary

Because of the large number of cells to be analyzed in cases of overexposure to ionizing radiation, an automated imaging system is desirable for scoring both translocations and dicentrics. This system should include three essential steps: automatic metaphase finding, automatic image capture at high magnification, and, finally, optimized data analysis for aberration interpretation. We evaluated a new image analysis system (CYTOGEN, IMSTAR, France) and found that its metaphase finder saved time, as much as quadrupling the speed of scoring chromosomal aberrations. Automatic metaphase selection did not appear to induce bias. We confirmed the equivalence of observing aberrations on a screen after automatic image capture and direct observation under a microscope. This work validated all of the steps necessary for obtaining images for automatic chromosomal aberration detection. The protocols for the detection of translocations may now be applied for biological dosimetry. This step will be validated in a future study.

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