SharePoint
Aide
IRSN, Institut de radioprotection et de sûreté nucléaire

Search our site :

ok

Contact us :

ok
En Fr

Enhancing Nuclear Safety


Research

Publications

Clearance of radiation-induced apoptotic lymphocytes: ex vivo studies and an in vitro co-culture model

Radiat Res 158(4), 464-74, 2002 Benderitter M, Durand V, Caux C, Voisin P.

Document type > *Article de revue

Keywords > molybdenum, irradiation

Research Unit > IRSN/DRPH/SRBE/LDB

Authors > BENDERITTER Marc, VOISIN Philippe

Publication Date > 01/10/2002

Summary

Lymphocytes are very sensitive to radiation. Our aim was to test the possibility of detecting apoptosis in lymphocytes as a potential short-term biomarker of ionizing radiation exposure. Our in vitro data confirmed the dose-time-effect relationships involved in radiation-induced apoptosis. The detection of in vivo induction of apoptosis in circulating lymphocytes after exposure of animals to radiation appears to depend critically on the technique used to measure apoptosis. Among the different techniques we investigated, mitochondrial modification was the most appropriate; they allowed establishment of dose-time-effect relationships when animals were observed for 72 h. A model of in vitro phagocytosis of apoptotic lymphocytes by macrophages was developed to mimic clearance of apoptotic cells occurring in vivo. Together, our data show that mitochondrial labeling may make it possible to detect ex vivo radiation-induced apoptosis of lymphocytes before macrophage ingestion occurs. We propose the measurement of apoptosis in lymphocytes as a potential short-term biomarker of ionizing radiation exposure. adress : Institut de Radioprotection et de Surete Nucleaire Departement de Protection de la sante de l'Homme et de Dosimetrie Laboratoire de Dosimetrie Biologique Multiparametrique F-92265 Fontenay-aux-Roses Cedex, France.

Close

Send to a friend

The information you provide in this page are single use only and will not be saved.
* Required fields

Recipient's email:*  

Sign with your name:* 

Type your email address:*   

Add a message :

Do you want to receive a copy of this email?

Send

Cancel

Close

WP_IMPRIMER_TITLE

WP_IMPRIMER_MESSAGE

Back

Ok