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La Recherchev2

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Molecular cloning of a new member of the p53 family from the pacific oyster Crassostrea gigas and seasonal pattern of its transcriptional expression level


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Titre de la revue : Marine Environmental Research Volume : 66 N° : 2 Pagination : 300-308 Date de publication : 01/08/2008

Résumé

Like other sessile filtrating molluscs, oyster may be exposed in the natural environment to a variety of contaminant. Long-term exposure to pollutants can lead to cancerous-like disorders, such as neoplasia. Environmentally induced alterations in p53 protein expression, in relation to leukemia, have been reported in various mollusc species inhabiting polluted water, suggesting that p53 proteins can also be used as a marker for environmental research. This work reports the cloning and sequencing of a p53-like cDNA in the mollusc bivalve Crassostrea gigas. The deduced amino acid sequences of p53 shared a high degree of homology with the homologues from other mollusc species, including typical eukaryotic p53 signature sequences. We examined the p53 transcription expression pattern during 18 months in oyster gills and whole soft tissues in four locations along the French coasts. Real-time PCR analysis indicated that p53 mRNA level display strong variations synchronized with the seasonal cycle in the four investigated locations
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