Efficacy of 3,4,3-LIHOPO for reducing neptunium retention in the rat after simulated wound contamination
AUTHORS : Paquet F, Montegue B, Ansoborlo E, Henge-Napoli MH, Houpert P, Durbin PW, Raymond KN
Int.J.Radiat.Biol. 2000, vol 76 n°1, 113-117
The ligand was tested for Np removal after intramuscular injection of Np-237 nitrate in rats.
Two experiments were performed, one with simultaneous injection of Np and LIHOPO at dosages ranging from 3 to 300 µmo/kg and the other with delayed administration of LIHOPO 30 µmol/kg from 5 to 30 min after Np injection.
The data obtained after simultaneous injections showed that the ligand dosage effectiveness was not linear and depended on the tissues beeing considered. For bones, the best results were obtained with 200 µmol/kg LIHOPO, where retention was reduced to 11% of controls. Maximum efficacies for removal in kidneys and liver were obtained with 30 µmol/kg LIHOPO where retention was reduced to 39% and 1.6% of controls, respectively. At higher dosages, LIHOPO seemed to have a reverse effect on these tissues, demonstrated by a significant accumulation of the radionuclide. The delayed edministration of LIHOPO dramatically decreased its efficacy. When administered 5 min after Np, LIHOPO was still efficient (60%, 37%, 7% of controls in bone, liver and kidneys, respectively), but not when treatment was delayed to 30 min.
The results demonstrated that LIHOPO was able to complex Np at the wound site but not after translocation to blood.