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In vitro differentiation and proliferation of purified human thymic and bone marrow CD7+CD2- T-cell precursors


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Mossalayi MD, Dalloul AH, Bertho JM, Lecron JC, Goube De Laforest P, Debre P.
Experimental Hematology Volume 18, Issue 4, 1990, Pages 326-331

Type de document > *Article de revue

Mots clés > radioprotection, radiohématologie, interleukine, thymus

Unité de recherche > IRSN/DRPH/SRBE/LTCRA

Auteurs > BERTHO Jean-Marc

Date de publication > 01/04/1990

Résumé

It is well established that CD7, gp40 antigen is one of the first antigens detected on the surfaces of cells of the human T-cell lineage. Using complement-dependent cytotoxicity and immunoadherence to anti-CD7-coated surfaces, we were able to purify CD7+2-3-4-8- TcR- cells with >90% purity from both human thymus and bone marrow. Limiting dilution analysis showed that these cells displayed high ability to generate mature T-cell clones when they were cultured in the appropriate conditions. These precursors needed phytohemagglutinin-stimulated leukocyte-conditioned medium (PHA-LCM) as a differentiation signal before being able to respond to PHA and recombinant interleukin 2 (rIL2). CD7+CD2- precursors differed from more mature CD7+CD2+ thymocytes because they were not sensitive to PHA, IL2, or CD2 triggering. Bone marrow-derived clones were mostly CD4+, whereas thymic cells generated more CD8+ than CD4+ clones. Together, this study indicates that the CD7+CD2- precursor is one of the earliest prothymocytes able to differentiate and proliferate in vitro.