Influence of Endothelial Cells on Vascular Smooth Muscle Cells Phenotype after Irradiation Implication in Radiation-Induced Vascular Damages
Fabien Milliat, Agnes Francois, Muriel Isoir, Eric Deutsch, Radia Tamarat, Georges Tarlet, Azeddine Atfi, Pierre Validire, Jean Bourhis, Jean-Christophe Sabourin,and Marc Benderitter. Am J Pathol,169:1484–1495, 2006.
Damage to vessels is one of the most common effects of therapeutic irradiation on normal tissues. We undertook a study in patients treated with preoperative
radiotherapy and demonstrated in vivo the importance of proliferation, migration, and fibrogenic phenotype of vascular smooth muscle cells (VSMCs) in radiation-induced vascular damage. These lesions may result from imbalance in the cross talk between
endothelial cells (ECs) and VSMCs. Using co-culture models, we examined whether ECs influence proliferation, migration, and fibrogenic phenotype of VSMCs. In the presence of irradiated ECs, proliferation and migration of VSMCs were increased. Moreover, expressions of alpha-smooth muscle actin, connective tissue growth factor, plasminogen activator inhibitor type 1, heat shock protein 27, and collagen type III, alpha 1 were up-regulated in VSMCs exposed to irradiated ECs. Secretion of transforming growth factor (TGF)-1 was increased after irradiation of ECs, and irradiated ECs activated the Smad pathway in VSMCs by inducing Smad3/4 nuclear translocation and Smad-dependent promoter activation. Using small interferring
RNA targeting Smad3 and a TGF-RII neutralizing antibody, we demonstrate that a TGF-1/TGF-- RII/Smad3 pathway is involved in the fibrogenic phenotype of VSMCs induced by irradiated ECs. In conclusion, we show the importance of proliferation,
migration, and fibrogenic phenotype of VSMCs in patients. Moreover, we demonstrate in vitro that ECs influence these fundamental mechanisms involved in radiation-nduced vascular damages.