A new image analysis system for biological dosimetry by fluorescent in situ hybridization. Step 1: metaphase finder and automatic metaphase acquisition validation

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01/06/2001

Roy L, Durand V, Delbos M, Sorokine-Durm I, Soussaline F, Voisin P. J Radiat Res (Tokyo) 2001 Jun;42(2):165-77

Type de document > *Article de revue
Mots clés publication scientifique > dosimétrie biologique , FISH
Unité de recherche > IRSN/DRPH/SRBE/LDB
Auteurs > ROY Laurence , VOISIN Philippe

Because of the large number of cells to be analyzed in cases of overexposure to ionizing radiation, an automated imaging system is desirable for scoring both translocations and dicentrics. This system should include three essential steps: automatic metaphase finding, automatic image capture at high magnification, and, finally, optimized data analysis for aberration interpretation. We evaluated a new image analysis system (CYTOGEN, IMSTAR, France) and found that its metaphase finder saved time, as much as quadrupling the speed of scoring chromosomal aberrations. Automatic metaphase selection did not appear to induce bias. We confirmed the equivalence of observing aberrations on a screen after automatic image capture and direct observation under a microscope. This work validated all of the steps necessary for obtaining images for automatic chromosomal aberration detection. The protocols for the detection of translocations may now be applied for biological dosimetry. This step will be validated in a future study.