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Feasibility and limits of bone marrow mononuclear cell expansion following irradiation



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Bertho JM, Mathieu E, Lauby A, Frick J, Demarquay C, Gourmelon P, Gorin NC, Thierry D.
International Journal of Radiation Biology - Volume 80, Issue 1, January 2004, Pages 73-81

Type de document > *Article de revue

Mots clés > radioprotection, interleukine, irradiation

Unité de recherche > IRSN/DRPH/SRBE/LTCRA

Auteurs > BERTHO Jean-Marc, DEMARQUAY Christelle, FRICK Johanna, GOURMELON Patrick, THIERRY Dominique

Date de publication > 01/01/2004


Purpose: To define the ability of bone marrow mononuclear cells (BMMNC) to expand after irradiation and to determine the amount of apoptosis in irradiated expanded cells. Materials and methods: Non-human primate BMMNC were irradiated in vitro at doses ranging from 0 to 4 Gy and were cultured during 1 week in the presence of interleukin 3, interleukin 6, stem cell factor, thrombopoietin and fms-like tyrosine kinase-3 ligand. The expansion yield of BMMNC, colony-forming cells and CD34+ cells were compared with non-irradiated control cultures. Apoptosis in expanded cells was also defined by annexin V/propidium iodine staining. Results: Irradiation of BMMNC up to 1 Gy did not modify the ability of haematopoietic cells to expand. At higher doses, expansion of haematopoietic cells is reduced as compared with non-irradiated cultures but it remains significant. This reduction in expansion of BMMNC was related to radiation-induced apoptosis. Conclusion: The results suggest that it is possible to expand haematopoietic cells after irradiation doses at least up to 2 Gy. This suggests a possible use of cell therapy for the treatment of radiation accident victims.