Follow-up of stable chromosomal aberrations in gamma-ray irradiated non-human primates

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01/07/2006

Titre de la revue : International Journal of Radiation Biology
Volume : 82
N° : 7
Pagination : 493-502 
Date de publication : 01/07/2006
Type de document > *Article de revue
Mots clés publication scientifique > dosimétrie biologique , dosimetrie rétrospective , primates non humains , translocation
Unité de recherche > IRSN/DRPH/SRBE/LDB
Auteurs > BERTHO Jean-Marc , DELBOS Martine , DEMARQUAY Christelle , GREGOIRE Eric , JACQUET Nelly , ROY Laurence , SOROKINE-DURM Irène , VOISIN Philippe

Purpose: The purpose of this study was to examine a new approach to retrospective biological dosimetry, by using a long-term animal model to determine the stability of translocation frequency after in vivo irradiation. While the frequency of dicentrics is known to decrease over time, the persistence of more stable chromosomal aberrations such as translocations could be useful if their stability were definitively proved. Materials and methods: Four monkeys ( Macaca fascicularis ) were exposed to two different doses of ionizing radiation: 2 Gy whole body irradiation for two and 4 Gy for two others. Blood samples were obtained at various times after irradiation. Both total and two-way translocations were detected by fluorescence in situ hybridization. Translocations were scored in stable cells, that is, those without dicentrics, rings or fragments. The course of translocation frequency was analysed at four time-points: one hour (H1), 2 months (M2), 10 months (M10) and 31 months (M31) after irradiation. Results: We observed two separate trends in translocation frequency: Total translocation frequency decreased slightly in animals irradiated with a dose of 2 Gy, while two-way translocation frequency was relatively stable in all irradiated animals. Conclusions: We confirmed the long-term stability of translocations and found that it seems to depend on the type of the translocation recorded. Overall translocations were stable for up to 31 months regardless of dose, but two-way translocations were more stable than those that were non-reciprocal, especially in stable cells.

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